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KMID : 0364820190550010017
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Korean Journal of Microbiology
2019 Volume.55 No. 1 p.17 ~ p.24
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Novel insight into the role of thiamine for the growth of a lichen-associated Arctic bacterium, Sphingomonas sp., in the light
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Pham Nhung
Pham Khoi
Lee Chang-Woo
Jang Sei-Heon
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Abstract
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Bacteria in the polar region are under strong light and ultravioletradiation. In this study, we investigated the effects of light onthe growth of a psychrophilic bacterium, Sphingomonas sp. PAMC 26621, isolated from an Arctic lichen Cetraria sp. Thegrowth of the strain in the light was lower than that in the dark. Surprisingly, thiamine increased the growth of Sphingomonassp. PAMC 26621 in M9 minimal medium under light conditions. Thiamine increased the growth of the strain in a concentrationdependentmanner along with ascorbic acid. N-acetylcysteinehad no effect on the growth of the strain in the light. Thiamineand ascorbic acid also increased the activities of glucose-6-phosphate dehydrogenase and superoxide dismutase. The resultsof this study indicate that thiamine provided by the lichensymbiosis system plays an important role in light-inducedoxidative stress in this Arctic bacterium as an antioxidant. Ourstudy provide insight into the biochemistry and physiology ofArctic bacteria under strong light and ultraviolet radiation.
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KEYWORD
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Sphingomonas sp. PAMC 26621, ascorbic acid, light, NADPH, thiamine
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